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Diagnostics

Archival cytologic samples suitable for PCR testing

Published in TB and Outbreaks Week, November 23rd, 2004

Archival cytologic samples are suitable for diagnostic PCR analysis.

"Use of the polymerase chain reaction (PCR) for the detection of B- and T-cell clonality, Epstein-Barr virus (EBV) and human herpesvirus 8 (HHV 8) infection is gaining increasing importance as a diagnostic modality," pathologists in the United States explained. "These tests are usually performed on fresh specimens."

"There are instances when fresh material is not available and there is a clinical utility for the performance of PCR on archival material via slide scrape lysates (SSL)," noted R. Mattu and colleagues at the National Cancer Institute. "However, the suitability of...

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