Diagnostics
LightCycler PCR assay detects/characterizes enterotoxigenic Escherichia coli
Published in TB and Outbreaks Week, November 30th, 2004
According to scientists in Germany, "To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness.
"Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and the type I heat-stable enterotoxin (ST 1) genes, using the LC hybridization probe format. A duplex assay for ST I with two...
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