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Norovirus Detection

LightCycler real-time RT-PCR assay rapidly detects/quantitates norovirus

Published in TB and Outbreaks Week, January 4th, 2005

A LightCycler real-time RT-PCR assay rapidly detects and quantitates norovirus.

"We developed an assay for the detection and quantitation of norovirus with the LightCycler SYBR Green-based real-time reverse transcription-PCR (real-time LC RT-PCR) and previously published primers in the capsid and the polymerase gene.

"One hundred thirty-two stool specimens from the Provincial Laboratory for Public Health (Microbiology), Alberta, Canada, and the Centers for Disease Control and Prevention, Atlanta, GA, were used to validate the new assay. The samples were collected from patients involved in outbreaks of acute gastroenteritis or children who presented...

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