Bacteriology
Transposon mutants and essential genes mapped in Helicobacter pylori
Published in TB and Outbreaks Week, February 1st, 2005
In a recent study, scientists in Seattle "constructed a genome-saturating mutant library of the human gastric pathogen" H. pylori.
"Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363 transposon mutants in our library," explained N.R. Salama and coauthors at the Fred Hutchinson Cancer Research Center. "While we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes."
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