Tuberculosis
Researchers lay foundation for gene therapy in tuberculosis
Published in TB and Outbreaks Week, April 12th, 2005
In a recent issue of the Journal of Medical Microbiology, W. Ju and associates at Wuhan University in China reported, "The purpose of this study was to express the HSP65 gene of Mycobacterium tuberculosis in eukaryotic cells and study its primary immune effect in animals. The HSP65 gene was amplified from the H37Rv strain of M. tuberculosis by PCR and then inserted into the expression plasmid pcDNA3.1-.
"The recombinant plasmid pcHSP65 was transfected into HeLa cells by using the liposome transfection method and also injected into BALB/C mice to accomplish DNA...
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