Mycobacterium Tuberculosis Diagnostics
Real-time PCR rapidly detects Mycobacterium tuberculosis in clinical samples
Published in TB and Outbreaks Week, June 28th, 2005
"We present a sensitive and specific assay for reliable and flexible detection of members of the Mycobacterium tuberculosis complex (MTBC) in clinical samples.
"This real-time PCR assay, which uses the LightCycler 2.0 instrument and 100-mcL glass capillaries, can provide a result within 1 h after DNA extraction," scientists writing in the Journal of Clinical Microbiology report.
"The primers amplify a 206-bp fragment of the MTBC 16S rRNA gene. The sensor hybridization probe targets a region highly specific to members of the...
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