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Tuberculosis

RT-PCR primer sets available for evaluating immunological processes in pig TB

Published in TB and Outbreaks Week, June 28th, 2005

Reverse transciption-PCR primer sets are available for evaluating immunological processes in guinea pig tuberculosis (TB) models, researchers in Japan report.

"Guinea pigs are often used as an animal model of human TB. However, there are few methods available for pursuing the immunological processes involved in guinea pig TB," reported H. Yamada and associates, Japan Anti-Tuberculosis Association.

"In this study," the researchers explained, "we developed for the first time systematic reverse transcription (RT)-PCR for evaluation of guinea pig mRNA expression. RT-PCR primer sets were newly designed for detection of cytokines and inducible nitric oxide...

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