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Mycobacteriology

Decontamination methods affect recovery of mycobacteria from clinical specimens

Published in TB and Outbreaks Week, July 5th, 2005

Decontamination methods affect the recovery of mycobacteria from clinical specimens.

According to recent research from the Netherlands, "We compared the NaOH-N-acetyl cysteine (NaOH-NALC) and the sulfuric acid decontamination procedure in the detection of mycobacteria using the Mycobacteria Growth Indicator Tube (MGIT).

"In total 219 sputum specimens were collected from 142 Zambian patients and subjected to mycobacterial culture. One half of the specimen was decontaminated with NaOH-NALC and the other half was decontaminated with sulfuric acid."

"From the 438 samples, a total of 261 (60%) cultures yielded growth of mycobacteria,...

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