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Rabies

Fluorometric cell-ELISA quantifies rabies infection and heparin inhibition

Published in TB and Outbreaks Week, August 9th, 2005

A fluorometric cell-ELISA quantifies rabies infection and heparin inhibition.

According to recent research published in the Journal of Virological Methods, "The purpose of the present study was to implement a fluorometric method for detecting and quantifying viral antigens in human meduloblastoma cells infected by two types of fixed rabies virus (CVS-MB and CVS-BHK) and a street virus using a cell-enzyme linked immunosorbent assay (cell-ELISA) technique; alkaline phosphatase was used as the antibody-marker enzyme and 4-methyl-umbelliferyl-phosphate as the fluorogenic substrate."

"The system was used for detecting up to 1:10,000 viral...

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