Diagnostics

TaqMan-based 1-step PCR assay rapid and sensitive for norovirus detection

Published in TB and Outbreaks Week, August 16th, 2005

A TaqMan-based 1-step PCR assay is rapid and sensitive for the detection of norovirus.

According to a study from the United States, "Noroviruses (NoV), which are members of the family Caliciviridae, are the most important cause of outbreaks of acute gastroenteritis worldwide and are commonly found in shellfish grown in polluted waters.

"In the present study, we developed broadly reactive 1-step TaqMan reverse transcription (RT)-PCR assays for the detection of genogroup I (GI) and GII NoV in fecal samples, as well as shellfish samples."

"The specificity and sensitivity of all steps of the assays were systematically evaluated, and...

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Source: TB and Outbreaks Week (2005-08-16)

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