Published in TB and Outbreaks Week, August 23rd, 2005
According to a study from Canada, "Conventional reverse transcription-polymerase chain reaction (Con RT-PCR) assay to detect norovirus is a complex multistep procedure that requires gel electrophoresis as well as hybridization or sequencing to confirm the results.
"To develop and evaluate a multiplex real time RT-PCR (Mrt RT-PCR) assay that detect and quantify norovirus GI and GII with a single amplification and detection step, the primers and TaqMan probes for the Mrt RT-PCR were selected from the ORF1-ORF2 junction region. A total of 97...
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