Tuberculosis

ELISA more sensitive than immunoblotting for detection of tuberculosis antibody

Published in TB and Outbreaks Week, August 23rd, 2005

An ELISA is more sensitive than immunoblotting for the detection of tuberculosis antibody.

According to recent research published in the journal Current Science, "The seroreactivity of a cocktail of purified mycobacterial excretory-secretory (ES) antigens ES-31, ES-41 and ES43 was assessed by ELISA and immunoblotting in patients with pulmonary tuberculosis.

"The ES-31 antigen was isolated by affinity chromatography and ES-41 and ES-43 were isolated by fast protein liquid chromatography from Mycobacterium tuberculosis H37Ra culture filtrate."

"Seven of 27 pulmonary tuberculosis sera were not reactive to ES-31 antigen...

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Source: TB and Outbreaks Week (2005-08-23)

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