TB & Outbreaks Week
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Diagnostics
Multiplex PCR enhances specificity of M. avium subspecies paratuberculosis detection
January 17th, 2006
A multiplex PCR assay enhances the specificity of Mycobacterium avium subspecies paratuberculosis detection. According to a study from Switzerland, "There are specificity questions inherent in most of the current PCR systems that amplify the MAP IS900 sequence as an indicator for Mycobacterium avium subspecies paratuberculosis (MAP) presence clue to false positives associated with IS900-like sequences that exists in other Mycobacterium species besides MAP. "We developed a multiplex PCR system designed to enhance specificity for MAP detection in a single PCR reaction. This PCR assay coamplifies the mycobacterium species 16S rRNA...
Source: TB & Outbreaks Week (2006-01-17)
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