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Real-time PCR more sensitive than viral isolation for equine herpesvirus type 1 detection

Published in TB and Outbreaks Week, March 14th, 2006

Real-time PCR is more sensitive than viral isolation for the detection of equine herpesvirus type 1.

According to recently published research in the Journal of Virological Methods, "Equid herpesvirus 1 (EHV1) is a major disease of equids worldwide causing considerable losses to the horse industry. A variety of techniques, including PCR have been used to diagnose EHV1.

"Some of these PCRs were used in combination with other techniques such as restriction enzyme analysis (REA) or hybridisation, making them cumbersome for routine diagnostic testing and increasing the chances of cross-contamination. Furthermore, they involve the use of suspected...

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