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RT-PCR rapidly differentiates infectious bronchitis virus vaccine strains and field isolates

Published in TB and Outbreaks Week, April 18th, 2006

An RT-PCR assay rapidly differentiates infectious bronchitis virus vaccine strains and field isolates.

According to a study from Australia, "Rapid differentiation of vaccine strains of infectious bronchitis virus (IBV) from wild type strains would enhance investigations of disease outbreaks.

"This study aimed to develop a reverse transcription-polymerase chain reaction (RTPCR) assay to differentiate between Australian vaccine strains of IBV and field isolates."

"Procedure A fragment of 6.5 kilobases that contains the S, M and N genes was amplified by RT-PCR from ten different IBV strains, including vaccine strains and field...

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