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Mycobacterium Tuberculosis Diagnostics

Agar-gel-immunodiffusion and ELISA specific but not sensitive for MAP detection in sheep

Published in TB and Outbreaks Week, September 12th, 2006

The agar-gel-immunodiffusion test and ELISA are specific but not sensitive for the detection Mycobacterium avium subspecies paratuberculosis in sheep.

"Our objective was to estimate the sensitivity and specificity of the agar-gel-immunodiffusion test (AGID), the ELISA, and the skin test for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in sheep using Bayesian methods without a gold standard. Fourteen flocks (2,465 sheep) were used," scientists writing in the journal Veterinary Research report.

According to S. Robbe-Austerman and colleagues at the U.S. Department of Agriculture, "Five flocks (450 sheep)...

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