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Mycobacterium Tuberculosis Diagnostics
Real-time PCR quantifies expression of genes involved in latent Mycobacterium tuberculosis
September 19th, 2006
Real-time PCR is used to quantify the expression of genes involved in latent Mycobacterium tuberculosis infection. "Real-time RT-PCR was used to quantify the expression of genes possibly involved in Mycobacterium tuberculosis latency in in vitro and murine models. "Exponential and stationary phase (EP and SP) bacilli were exposed to decreasing pH levels (from 6.5 to 4.5) in an unstirred culture, and mRNA levels for 16S rRNA, sigma factors sigA, B, E, F, GH and M, Rv0834c, icl, nirA, narG fpbB, acr, rpoA, recA and cysH were quantified," scientists in Spain report. According to the authors, "The expression of acr was...
Source: TB & Outbreaks Week (2006-09-19)
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