Published in TB and Outbreaks Week, January 8th, 2008
"Previous estimates of the amount of WNV inoculated by mosquitoes (10(1.2) - 10(4.3) PFU) were based on in vitro assays that do not allow mosquitoes to probe or feed naturally. Here, we developed an in vivo assay to determine the amount of WNV inoculated by mosquitoes as they probe and feed on peripheral tissues of a mouse or chick. Using our assay, we recovered approximately one-third of a known amount of virus inoculated into mouse tissues....
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