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Studies in the area of life sciences reported from Wadsworth Center

Published in TB and Outbreaks Week, January 8th, 2008

"West Nile virus (WNV) is transmitted to vertebrate hosts by mosquitoes as they take a blood meal. The amount of WNV inoculated by mosquitoes as they feed on a live host is not known," investigators in the United States report.

"Previous estimates of the amount of WNV inoculated by mosquitoes (10(1.2) - 10(4.3) PFU) were based on in vitro assays that do not allow mosquitoes to probe or feed naturally. Here, we developed an in vivo assay to determine the amount of WNV inoculated by mosquitoes as they probe and feed on peripheral tissues of a mouse or chick. Using our assay, we recovered approximately one-third of a known amount of virus inoculated into mouse tissues....

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