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TB & Outbreaks Week

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Tuberculosis



Scientists at Nihon University publish research in tuberculosis



June 24th, 2008

According to recent research from Tokyo, Japan, "Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR."

"In this study, we attempted to improve the original QNRT-PCR assay and newly developed the wide-range QNRT-PCR (WR-QNRT-PCR) assay, which is more accurate and has a wider detection range. For use as an internal-control ''calibrator'' to measure the copy number of M. tuberculosis DNA, an original new-mutation plasmid (NM-plasmid)...


Source: TB & Outbreaks Week (2008-06-24)

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