TB & Outbreaks Week
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Tuberculosis
Recent findings from A. Amaro and co-authors highlight research in tuberculosis
July 29th, 2008
" To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide (CTAB) extraction," investigators in Lisbon, Portugal report. "The yield and quality of DNA were compared by spectrophotometry, agarose gel electrophoresis, restriction endonuclease analysis and PCR. The combined bead beating and enzymatic extraction method yielded more DNA. However, that method produced some sheared DNA, visible either by agarose gel electrophoresis or by...
Source: TB & Outbreaks Week (2008-07-29)
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