Burkholderia

Scientists at University of Hawaii target Burkholderia

Published in TB and Outbreaks Week, August 26th, 2008

According to recent research from the United States, "Allelic replacement in the Burkholderia genus has been problematic due to the lack of appropriate counter-selectable and selectable markers. The counter-selectable marker sacB, commonly used in gram-negative bacteria, is nonselective on sucrose in many Burkholderia species."

"In addition, the use of antibiotic resistance markers of clinical importance for the selection of desirable genetic traits is prohibited in the United States for two potential bioterrorism agents, Burkholderia mallei and Burkholderia pseudomallei. Here, we engineered a mutated counter-selectable marker based on the B. pseudomallei PheS (the...

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Source: TB and Outbreaks Week (2008-08-26)

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