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West Nile Virus
Research from Cornell University provide new insights into west nile virus
October 7th, 2008
According to recent research published in the Journal of Clinical Microbiology, "We have developed a novel multiplex reverse transcription-PCR ligase detection reaction (RT-PCR/LDR) assay for the detection of West Nile virus (WNV) in both clinical and mosquito pool samples. The method relies on the amplification of three different genomic regions, one in the coding sequence of nonstructural protein NS2a and two in nonstructural protein NS5, to minimize the risk of detection failure due to genetic variation." "The sensitivity of the PCR is complemented by the high specificity of the LDR step, and the detection of the LDR products can be achieved with capillary...
Source: TB & Outbreaks Week (2008-10-07)
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