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Plague
New plague research has been reported by B. Ni et al
November 11th, 2008
According to recent research published in the journal Letters in Applied Microbiology, "Plasmids are critical for the pathogenicity of Yersinia pestis. In order to carry out a systematic investigation of their role in pathogenesis, we cured plasmids from Y. pestis." "Each plasmid's replicon of Y. pestis was cloned into plasmid pEX18Gm containing a counter-selectable sacB gene, and was then introduced into Y. pestis strain 201 by electroporation. Strains containing recombinant plasmids were cultivated under antibiotic selection. The resultant plasmid-curing colonies, identified by specific polymerase chain reactions, were then cured off pEX18Gm under sucrose...
Source: TB & Outbreaks Week (2008-11-11)
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