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TB & Outbreaks Week

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Plague



Research from Centers for Disease Control and Prevention in plague provides new insights



November 11th, 2008

A new study, 'Detection of viable Yersinia pestis by fluorescence in situ hybridization using peptide nucleic acid probes,' is now available. According to a study from the United States, "A successful method has been developed for the detection of live Yersinia pestis, the plague bacillus, which incorporates nascent RNA synthesis. A fluorescent in situ hybridization (FISH) assay using peptide nucleic acid (PNA) probes was developed specifically to differentiate Y. pestis strains from closely related bacteria."

"PNA probes were chosen to target high copy mRNA of the Y. pestis caf1 gene, encoding the Fraction 1 (F1) antigen, and 16S ribosomal RNA. Among Yersinia strains...


Source: TB & Outbreaks Week (2008-11-11)

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