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Escherichia Coli
Reports from S.C. Dewet and co-researchers add new data to research in Escherichia coli
December 9th, 2008
In this recent study, researchers in Australia conducted a study "To develop a rapid RNA extraction procedure for maximizing bacterial RNA yield from carcass samples with low abundance of Escherichia coli O157:H7 without pre-enrichment. Nontarget bacterial cells were added to the sample prior to RNA extraction, facilitating the co-precipitation of target RNA along with nontarget RNA and thus enhancing the recovery." "This method was developed using a serial dilution of log phase target cells (E. coli O157:H7), combined with a high number of nontarget cells (E. coli K12). Cells were lysed by a bead beating method followed by RNA purification using a commercial kit. A...
Source: TB & Outbreaks Week (2008-12-09)
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