TB & Outbreaks Week
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Life Sciences
New life sciences study findings have been reported by scientists at University of Helsinki
January 20th, 2009
"We developed a real-time PCR protocol to detect orthopoxviruses (OPVs) from different clinical specimens and to separate variola virus from other OPVs. In our protocol, we used automated nucleic acid extraction system together with real-time PCR to create a simple, safe and fast procedure to obtain an initial result," researchers in Helsinki, Finland report. "The sensitivity was better by using designed hybridization probes as compared to SYBR green I for detection. The detection limit ranged from 13 to 1,300 copies per 20 mu l reaction volume depending on the sample type. The PCR detected all OPVs pathogenic to human (variola, cowpox, monkey-pox, vaccinia) as well as...
Source: TB & Outbreaks Week (2009-01-20)
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