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Legionnaire Disease
New legionnaire disease research from B. Chang and co-researchers described
February 3rd, 2009
"Legionella organisms are prevalent in manmade water systems and cause legionellosis in humans. A rapid detection method for viable Legionella cells combining ethidium monoazide (EMA) and PCR/real-time PCR was assessed," scientists writing in the journal Applied and Environmental Microbiology report. "EMA could specifically intercalate and cleave the genomic DNA of heat- and chlorine-treated dead Legionella cells. The EMA-PCR assay clearly showed an amplified fragment specific for Legionella DNA from viable cells, but it could not do so for DNA from dead cells. The number of EMA-treated dead Legionella cells estimated by real-time PCR exhibited a 10(4)- to...
Source: TB & Outbreaks Week (2009-02-03)
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