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TB & Outbreaks Week

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Tuberculosis



New tuberculosis findings from Y.L. Zhao and co-authors described



February 17th, 2009

"In-use carbol fuchsin stains were collected from 10 different routine acid-fast bacilli smear microscopy laboratories. To examine the variations in the composition of carbol fuchsin stains," scientists writing in the International Journal of Tuberculosis and Lung Disease report.

"Carbol fuchsin concentrations were first determined spectrophotometrically by measuring absorbance at 547 nm. High-performance liquid chromatography (HPLC) separated and quantified the four basic fuchsin homologues: para-rosaniline, rosaniline, magenta II and new fuchsin, and identity was confirmed by mass spectrometry (MS). Absorbance measurement showed that three of 10 (30%) samples...


Source: TB & Outbreaks Week (2009-02-17)

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