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DNA Research
Research from Wuhan University reveals new findings on DNA research
May 5th, 2009
"Simple and sensitive chemiluminescence (CL) and CL imaging methods have been developed for the magnetic bead-based DNA hybridization assay. The assay relies on the high sensitivity and long stable light signal of the CL system in which horseradish peroxidase (HRP) catalyzes the luminol-H2O2 reaction with para-iodophenol (PIP) as the enhancer," scientists in Wuhan, People's Republic of China report. "In this protocol, a sandwich DNA hybridization is performed by mixing the target DNA with the magnetic bead-captured DNA and the biotinylated reporter DNA, followed through the biotin-streptavidin reaction with conjugated HRP, and then the conjugated HRP is determined by...
Source: TB & Outbreaks Week (2009-05-05)
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