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TB & Outbreaks Week

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Polymerase Chain Reaction



Report summarizes polymerase chain reaction study findings from D. Pitapereira and co-researchers



June 2nd, 2009

"To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic periurban area of cutaneous leishmaniasis in Porto Alegre, Brazil," scientists writing in the American Journal of Tropical Medicine and Hygiene report.

"Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of. three positive pools with at least...


Source: TB & Outbreaks Week (2009-06-02)

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