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Tuberculosis
Data from T. Soejima and colleagues advance knowledge in tuberculosis
June 9th, 2009
"Flow cytometry (FCM) using propidium iodide (PI)/bis-oxonol (BOX) staining can distinguish live, dead, and sublethally injured Escherichia coli by detecting intact vs. nonintact membranes (PI) and membrane potential (BOX). However, live bacteria, especially Mycobacterium tuberculosis, are not likely to be successfully discriminated from injured bacterium by FCM when utilizing the live/dead staining agents currently on the market," scientists writing in the journal Fems Microbiology Letters report. "As injured cell membranes have integrity like that of live cells and are regarded as such by FCM, the distinction between live and injured cells has depended on the...
Source: TB & Outbreaks Week (2009-06-09)
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