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Life Sciences
Scientists at Department of Agriculture release new data on life sciences
July 7th, 2009
"A cell-free translation (CFT) assay for detecting Shiga toxin (Stx) at different levels of purity has been validated. The limits of detection for pure Stx2 (PStx2) and partially pure Stx2 (PPStx2) in water reached 20 and 3.5 pg/mu L, respectively, without the artificial process of proteolytic activation and reduction of the pro-toxin," investigators in the United States report. "The specific detection of Stx2 was confirmed by a neutralization test using Stx2-specific mouse monoclonal antibody. This assay can be used for differentiation of Stx-producing Escherichia coli from non-Stx-producing E coli. Four E coli O157:H7 strains genotypically different for Stx were...
Source: TB & Outbreaks Week (2009-07-07)
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