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Clinical Microbiology and Infectious Disease

Study results from G. Goy and colleagues broaden understanding of clinical microbiology and infectious disease

Published in TB and Outbreaks Week, January 5th, 2010

According to a study from Lausanne, Switzerland, "Waddlia chondrophila is considered as an emerging human pathogen likely involved in miscarriage and lower respiratory tract infections. Given the low sensitivity of cell culture to recover such an obligate intracellular bacteria, molecular-based diagnostic approaches are warranted."

"We thus developed a real-time PCR that amplifies Waddlia chondrophila DNA. Specific primers and probe were selected to target the 16S rRNA gene. The PCR specifically amplified W. chondrophila but did not amplify other related-bacteria such as Parachlamydia acanthamoebae, Simkania negevensis and Chlamydia pneumoniae. The PCR exhibited a good...

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