Published in Telemedicine Law Weekly, September 22nd, 2007
"NRRL 5646 efficiently catalyzes the decarboxylation of vanillic acid to the phenolic product, guaiacol. For purification and characterization of vanillic acid decarboxylase (VAD), a simple, rapid enzyme assay was devised to couple the vanillic acid decarboxylase reaction with horseradish peroxidase/H2O2.Guaiacol formed in the decarboxylation reaction is oxidized by peroxidase to tetraguaiacol, a chromophoric substance, which is measurable at 470 nm. Soluble, VAD activity was inducible in cells grown...
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