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Enzyme Research

Research results from K.H. Jung and co-authors update knowledge of enzyme research

Published in Telemedicine Week, January 19th, 2010

According to recent research from Seoul, South Korea, "Cell preparation procedures injurious to Na/I symporters (NIS) could deter their usefulness for reporter gene assays and in vivo cell imaging. in this study, we investigated the effects of cell collection by trypsinization on radioiodide transport and in vivo cell imaging results. The influence of trypsinization procedures on I-125 transport was evaluated using Huh-7/NIS hepatoma cells."

"The effects of graded concentrations of trypsin and EDTA were assessed on Huh-7/NIS and A431/NIS lung cancer cells. Trypsin-induced NIS proteolysis was investigated by immunoblots of plasma membrane prepared from...

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