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Stem Cell Transplantation

"Optimal Timing for Processing and Cryopreservation of Umbilical Cord Haematopoietic Stem Cells for Clinical Transplantation."

Published in Transplant Weekly, March 15th, 1999

According to the authors' abstract of an article published in Bone Marrow Transplantation, "Some of the factors that may influence the number and quality of cord blood haematopoietic progenitor cells available for transplantation have been investigated including site of collection, delayed processing after collection, and cryopreservation protocol. We used the granulocyte-macrophage progenitor (CFU-GM) and erythroid burst-forming unit (BFU-E) assays to quantify progenitors. The capacity of CFU-GM to produce secondary colonies was used as a measure of progenitor cell quality. We found that: (1) there were no significant differences in total nucleated cells (TNC), mononuclear cells...

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