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Mycobacteria Diagnostics

Slow growing Mycobacterium species directly identified by 16S-23S rDNA ISR analysis

Published in Tuberculosis Week, April 10th, 2006

Slow growing Mycobacterium species were directly identified by intergenic 16S-23S rDNA spacer region analysis.

According to recently published research from Australia, "To obtain Mycobacterium species identification directly from clinical specimens and cultures, the 16S-23S rDNA spacer (ISR) was amplified using previously published primers.

"The restriction enzyme that could potentially produce the most restriction fragment length polymorphisms (RFLPs) was determined from all available ISR DNA sequences in GenBank to produce a novel data set of RFLPs for 31 slowly growing Mycobacterium species."

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