"cDNA Cloning and Sequencing of Variable Region Gene of Monoclonal Antibody SZ-39 Specific for Human Brain Glioma."

According to an abstract submitted by the authors to the 4th International Conference on Human Antibodies and Hybridomas, held April 26-28, 1995, in Amsterdam, The Netherlands, "We have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable region gene by PCR. The primers incorporate restriction sites that allow the cDNA of the variable domain to be force cloned for sequencing and expression. Here we have applied the technique to clone and sequence the heavy and light chain variable domain of monoclonal antibody that reacts with human brain glioma line SHG-44. Total RNA were isolated from SZ-39 hybridoma cells and NS-1...

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