"Improved Suicide Vectors for Chromosomal Mutagenesis in Salmonella typhi."

According to an abstract submitted by the authors to the 95th General Meeting of the American Society for Microbiology, held May 21-25, 1995, in Washington, D.C., "A common technique for mobilizing deletion-inactivated genes into the chromosome of a host strain involves the use of 'suicide vectors.' These vectors replicate autonomously in permissive backgrounds, but integrate by homologous recombination into the chromosome of nonpermissive host strains, resulting in an unstable merodiploid state; merodiploids can then spontaneously resolve, leaving an inactivated copy of the target gene within the chromosome. Two suicide vector systems currently in use are derived from the parental...

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