Pediatrics (Pertussis)
"Validation of Nested Bordetella PCR in Pertussis Vaccine Trial."
Published in Vaccine Weekly, May 6th, 1996
According to the authors' abstract of an article published in Journal of Clinical Microbiology, "A nested PCR, using a 239-bp sequence in the pertussis toxin promoter region, was developed and evaluated. The assay differentiates Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica by restriction enzyme analysis of the amplified fragments. The diagnostic performance of the PCR was evaluated in a Swedish pertussis vaccine efficacy trial which took place from 1992 to 1995, including study children and household members and using culture and serology for laboratory confirmation of suspected cases. In total 2,421 nasopharyngeal aspirates were...
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Source: Vaccine Weekly (1996-05-06)
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