"Characterization of the Nucleotide Sequence of the groE Operon Encoding Heat Shock Proteins Chaperone-60 and -10 of Francisella tularensis and Determination of the T-Cell Response to the Pro

According to the authors' abstract of an article published in Infection and Immunity, "The groE operon of Francisella tularensis LVS, encoding the heat shock proteins chaperone-10 (Cpn10) and Cpn60, was sequenced and characterized, and the T-cell response of LVS-vaccinated individuals to the two proteins and the third major chaperone, Ft-DnaK, was assayed. The cpn10 and cpn60 genes were amplified by PCR with degenerate oligonucleotides derived from the N-terminal sequence of the two proteins. The sequence analysis revealed the expected two open reading frames, encoding proteins with estimated M(r)s of 10,300 and 57,400. The deduced amino acid sequences closely resembled cpn10 and...

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