Published in Vaccine Weekly, January 22nd, 2003
M. Kawahara and colleagues set out to do this, first developing "a quantitative assay using a real-time reverse transcription-PCR (RT-PCR) to measure guinea pig gamma interferon (IFN-gamma), interleukin-12 (IL-12), IL-10, and transforming growth factor beta (TGF-beta) mRNA."
"By preparing primer-fluorogenic probe sets for these cytokines and standard RNA templates corresponding to...
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