HIV Genetics

Genome sequences of three South African HIV type 1 subtype C isolates characterized

Published in Vaccine Weekly, April 16th, 2003

The near-full length genome sequences of three South African human immunodeficiency virus (H(V) type 1 subtype C isolates have been characterized.

G.M. Hunt and colleagues at the University of Witwatersrand, National Institute of Communicable Diseases, AIDS Virus Research Unit, isolated "proviral DNA ... from primary cultures of three South African R5 isolates and the near-full length genome amplified by PCR [polymerase chain reaction]."

They cloned the PCR product "into the pCR-XL-TOPO vector and a representative clone from each isolate sequenced by primer walking," the researchers explained.

"Phylogenetic analysis showed all...

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Source: Vaccine Weekly (2003-04-16)

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