Xylanase

Cell wall protein used as fusion partner for expression of xylanase A

Published in Vaccine Weekly, April 27th, 2005

Use of the cell wall protein pir4 as a fusion partner for the expression of Bacillus species BP-7 xylanase A in Saccharomyces cerevisiae

"Xylanase A from Bacillus species BP7, an enzyme with potential applications in biotechnology, was used to test Pir4, a disulfide bound cell wall protein, as a fusion partner for the expression of recombinant proteins in standard or glycosylation-deficient mnn9 strains of Saccharomyces cerevisiae. Five different constructions were carried out, inserting in-frame the coding sequence of xynA gene in that of PIR4, with or without the loss of specific regions of PIR4," scientists in Spain report.

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Source: Vaccine Weekly (2005-04-27)

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