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Protein Production
Recombinant protein produced using HeLaS3-vaccinia virus system
September 14th, 2005
Scientists have produced recombinant protein using the HeLaS3-vaccinia virus expression system. According to recent research from the United States, "Adaptation of the vaccinia virus expression system to HeLa S3 suspension bioreactor culture for the production of recombinant protein was conducted. Evaluation of hollow fiber perfusion of suspension culture demonstrated its potential for increased cell density prior to infection." "The hollow fiber was also used for medium manipulations prior to infection. Two process parameters, multiplicity of infection (MOI) and temperature during the protein production phase, were evaluated to determine their...
Source: Vaccine Weekly (2005-09-14)
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