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HIV/AIDS Vaccine

Method to produce HIV-1 negative factor protein has been developed

Published in Vaccine Weekly, August 9th, 2006

Scientists have developed a method to produce recombinant HIV-I negative factor (Nef) protein using Pichia pastoris and a low-temperature fed-batch strategy.

According to recent research published in the journal Biotechnology and Applied Biochemistry, "In the present paper we describe the cloning and extracellular expression of the HIV-1 Nef (negative factor) protein utilizing the yeast Pichia pastoris, as well as the successful use of a low-temperature fed-batch strategy for decreasing end-product degradation by proteases."

"The nef gene in a pPICZ alpha A vector was integrated into the genome of three different P....

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