Published in Vaccine Weekly, August 9th, 2006
"A system based on reverse transcription polymerase chain reaction (RT-PCR) of the RNA genonne was established to identify genetic composition of influenza viruses generated by reassortment between an attenuated donor virus and virulent wild type virus," wrote S.H. Ha and colleagues, Yonsei University.
"The primers were designed, by multiple sequence alignment of variable regions, specific for cold-adapted donor virus HTCA-A101, as compared to other influenza A viruses. The specificity of each primer set was...
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