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Life Sciences
Study findings on life sciences are outlined in reports from R. Sellek and colleagues
May 7th, 2008
A report, 'Recovery of Francisella tularensis from soil samples by filtration and detection by real-time PCR and cELISA,' is newly published data in Journal of Environmental Monitoring. "The aim of this study was to develop a specific and highly sensitive method able to detect very low concentrations of Francisella tularensis in soil samples by real-time PCR (qPCR) with SYBR Green I. tul4 gene, which encodes the 17-kDa protein (TUL4) in F. tularensis strains, was amplified using a LightCycler (LC) device. We achieved a detection limit of 0.69 fg of genomic DNA from F. tularensis subp. holarctica live vaccine strain (LVS), corresponding to a value less than 3.4 genome equivalents per...
Source: Vaccine Weekly (2008-05-07)
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