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Porcine Reproductive And Respiratory Syndrome
New porcine reproductive and respiratory syndrome study results reported from Y.H. Hou et al
May 14th, 2008
"We transfected a recombinant plasmid that co-expressed swine ubiquitin and a codon optimized GP5 encoding-gene of porcine reproductive and respiratory syndrome virus (PRRSV), designated pCA-U-optiGP5, as well as the plasmid pCA-optiGP5 encoding codon optimized GP5, and the plasmid pCA-GP5 expressing wild-type pGP5 into 293T cells. Expression of GP5 was measured by indirect immunofluorescence (1117) assay and Western blot and found to be considerably higher in response to pCA-U-optiGP5 than the wild-type vector," scientists in Harbin, People's Republic of China report. "GP5 protein was rapidly degraded in pCA-U-optiGP5-transfected 293T cells. The proteasome inhibitor,...
Source: Vaccine Weekly (2008-05-14)
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