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Vaccine Weekly

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DNA Vaccines



Research from Southeast University in the area of DNA vaccines published



June 4th, 2008

According to recent research from Nanjing, People's Republic of China, "In this paper, we addressed the immune adjuvant effects of interleukin(IL)-21 on DNA vaccine constructs expressing mycobacterium tuberculosis (TB) Ag85A and compared immune responses induced in mice inoculated DNA vaccine constructs expressing Ag85A and IL-21 with mice inoculated DNA vaccine constructs expressing Ag85A alone or Bacillus Galmette-Guerin(BCG.). In this experiment, the gene of IL-21 was firstly amplified from plasmid pcDNA3.1-mIL21 by PCR and cloned into the plasmid pRSC, forming recombinant plasmid pRSC-IL21."

"Then, the gene of Ag85A was amplified from the plasmid pIRES-Ag85A by PCR...


Source: Vaccine Weekly (2008-06-04)

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